FIG 3.

Cells expressing mCD134 harboring a double amino acid mutation are permissive for HHV-6B infection. (A) JJHan cells were transduced with nonreplicative lentiviruses expressing mCD134 or its mutants for 24 h. A portion of the cells were stained with an FITC-conjugated anti-mCD134 antibody at 4°C for 1 h. Then, the cells were subjected to the surface expression analysis of mCD134 and its mutants by flow cytometry (EC800; Sony). (B and C) The remaining cells were infected with HHV-6B (HST strain) and harvested at 24 h postinfection. A portion of the cells were fixed with acetone-methanol (7:3) and stained with anti-IE1 antibody and secondary antibody for IFA (B). The remaining cells were lysed and examined by Western blotting with anti-IE1 and anti-α-tubulin antibodies (C). JJHan cells transduced with nonreplicative lentiviruses expressing no alien protein were used as a control for these experiments. Numbers at the left of the gels are molecular masses (in kilodaltons).