FIG 2.

Identification of Sam68 binding sites in the EV71 IRES. (A) The secondary structure of the 5′ UTR was predicted by MFold software (47). The first and the last nucleotides in each stem-loop are numbered as indicated. Stem-loops I to VI are shown. (B) A serial deletion to eliminate each of the six stem-loops of the IRES was constructed. RNA probes were labeled with [α-³²P]UTP (0.2 × 10⁴ to 1 × 10⁴ cpm per reaction mixture), and an EMSA was performed as described in the legend to Fig. 1A. Sam68 interacts with stem-loops IV and V, but it did not bind to other regions of the IRES.