FIG 5.

Sam68 overlaps with PCBP2 and PABP. Mock-infected and EV71-infected HeLa cells at 5 hpi were simultaneously probed with mouse anti-PCBP2 (A), mouse anti-PABP (B), and rabbit anti-Sam68 antibodies, followed by goat anti-rabbit secondary antibodies conjugated to FITC (green) and goat anti-mouse secondary antibodies conjugated to TRITC (red). Nuclear material was stained with DAPI (blue). (C) Sam68 coprecipitates with PCBP2 and PABP. Mock-infected HeLa cells or cells infected with EV71 at an MOI of 10 were harvested at 1, 2, 4, 6, and 12 hpi. Whole-cell lysates (WCL) were immunoprecipitated (IP) with agarose beads coupled to anti-Sam68 antibody (α-Sam68). Bound protein was eluted using a low-pH solution (pH 2.5), and the collected samples were analyzed by Western blotting (WB) probing with anti-PCBP2 and anti-PABP.