FIG 7.

Effects of downregulation and upregulation of Sam68 on EV71 replication. (A) HeLa cells and RD cells were transfected with either Sam68 shRNA, control shRNA, or no RNA for 72 h and then infected with EV71 at an MOI of 1 for 24 h. The concentrations of endogenous Sam68 and virus VP1 in each sample were evaluated by Western blotting probing with anti-Sam68 and anti-EV71 VP1 antibody. The Western blot was also probed with anti-β-actin antibody to normalize loading between lanes. The supernatants were collected, and the virus titers were determined using the plaque assay previously described. (B) HeLa cells and RD cells were transfected with either Flag-EV or Flag-Sam68 or without Flag for 48 h and then infected with EV71 (MOI of 1) for 24 h. The expression levels of Sam68 and EV71 VP1, as well as the virus titers, were determined as described above for panel A.