FIG 1.

Identification of virus entry inhibitors specific to filovirus. (a) The Prestwick library of 1,200 FDA-approved drugs was screened against pseudotyped Marburg virus entry in the A549 cell line. From the primary screen, 20 compounds were identified and validated with pseudotyped H5N1 influenza virus (AIV, blue), Ebola virus (EBOV, green), and Marburg virus (MARV, red). Error bars represent standard deviations (n > 3). The compounds and their respective targeted GPCRs are shown below the graph. (b) Function enrichment analysis of the 20 filovirus entry inhibitors was performed to evaluate if the GPCR antagonism function was enriched by chance. The mechanism of action information of each identified entry inhibitor and every Prestwick library compound was extracted from the drug information sheet provided by the Prestwick library, and data were compared and tested for overrepresentation using a Fisher exact test. The significance threshold is indicated by a dotted line at 1.3, which represents −log(P value) (where P = 0.05).