FIG 3.
Western blot of BNLF2a expression in untreated Akata cells (Un), Akata cells treated with anti-human IgG for 24 h (In), the EBV-negative gastric cancer cell line AGS, and the EBV-positive gastric cell lines SNU719, NCC24, and YCCEL1. BNLF2a, BZLF1, and GAPDH were detected using the rat anti-BNLF2a monoclonal antibody 5F8 (a generous gift from Elizabeth Kremmer), a mouse anti-ZEBRA monoclonal antibody (catalog no. sc-53904; Santa Cruz Biotechnology), and a goat anti-GAPDH polyclonal antibody (catalog no. GTX100118; GeneTex), respectively.