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Immunofluorescence analysis of the tight junction protein ZO-1 and the cilium marker β-tubulin IV of infected HAE-ALI cultures. At 14 days p.i., mock-, WT-, ΔNS2-, and ΔNS3-infected HAE-ALI cultures were costained with anti-NS1C and anti-β-tubulin IV antibodies (A) or with anti-NS1C and anti-ZO-1 antibodies (B). Confocal images were taken at a magnification of ×40. Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) (blue).
