FIG 10.
IN in trans stimulates WT core formation in ΔIN virions. (A) Infectivity of HIV-Luc.ΔIN transcomplemented with Vpr-INWT or Vpr-INH171T made in the presence of BI-D or DMSO. Values were normalized to control HIV-Luc virus constructed in the presence of DMSO. (B) (Upper) Quantitation of core morphology frequencies of 100 counted virions (the ΔIN mutation yields more immature particles than does other class II [e.g., missense] mutations [18, 46]). Shown are averages ± SD for two independent experiments for the transcomplemented preparations described for panel A. (Lower) Representative images of the mature, eccentric, and immature virions observed within experiments.