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. 2015 Jul 15;89(19):9765–9780. doi: 10.1128/JVI.01522-15

FIG 6.

FIG 6

Tomo-bubblegrams of immature HIV-1 particles. (Ai to Avi) Central tomographic sections (left) and corresponding tomo-bubblegram sections (right). Full tomograms of the virions in panels Aii (Movie S5), Aiii (Movie S6), and Avi (Movie S7) are shown in movies in the supplemental material. The diagrams on the left side of the figure illustrate the composition of each sample. PR− particles contain the Gag and Gag-Pol polyproteins that include NC and incorporate vRNA. The virions are immature due to the inactivating D25A mutation in the PR active site. Gag/ψ-minus particles are also immature, and additionally their RNA lacks the ψ region and therefore vRNA is not incorporated into particles. The Gag-LeuZip particles replace NC with a leucine zipper; these particles lack NC and vRNA. Bar, 50 nm. (B) Total virion RNA from the particles described for panel A. RNA concentration was normalized to the Gag signal obtained from anti-p24 Western blots of multiple input volumes. Shown are the averages (±SD) of total RNA (in nanograms) standardized by Gag signal for four independent experiments, reported as a percentage of PR−. (C) RNA from panel B was utilized to determine the copy number of vRNA. Values, reported as a percentage of PR−, are the averages (±SD) for four independent experiments.