FIG 7.

Locations of bubbles in PR⁻ virions. (A) Cryo-ET central slice of an immature PR⁻ particle. (B and C) Tomo-bubblegram section corresponding to panel A. In panel C, bubbles are marked with red circles. (D) Surface rendering of a cutaway slice of the particle shown in panels A to C. Most of the bubbles (white) are located between the CA layer (green) and the NC-vRNA layer (yellow). The viral envelope is in cyan, and the internal contents are in red. Bar, 50 nm. (E) Radial density profile of subtomogram averaged Gag from immature virions (from reference 25). (F) Histogram of the distance from the center of the bubbles to the center of the viral envelope (membrane + MA).