FIG 8.

Bubblegram imaging of purified NC protein. (A to E) Cryo-EM dose series of five NC aggregates of different sizes. The images in which bubbles first appear are labeled with a white asterisk in each case (although NC is formally soluble, small aggregates of suitable size were consistently found on EM grids). Bar, 50 nm. (F) Plot of the diameters of NC aggregates versus the cumulative electron dose at which they started bubbling. Points corresponding to the aggregates from panels A to E are shown in red. While the data exhibit some stochastic variability in bubbling threshold, there is a clear trend toward larger aggregates requiring less electron radiation to initiate bubbling. A linear fit is shown for reference (red line).