FIG 9.

Viral RNA requirement for eccentric condensate formation. (A) Total RNA (in nanograms) of concentrated virion preparations. RNA concentration was standardized by the p24 signal obtained from Western blots of multiple input volumes. Shown are averages (±SD) from four independent experiments reported as percentages of untreated HIV-Luc virions. (B) Average (±SD) copies of vRNA per ng total RNA (n = 4) determined for the samples described for panel A. (C) (Left) Core morphology frequencies in sets of 100 counted virions (average ± SD) from two independent isolates of particles prepared as described for panel A. **, P < 0.01; n.s., not significant (P = 0.37). (Right) Representative images of the mature, eccentric, dual electron density (ED), empty, and immature virions observed in these experiments.