FIG 4.

Soluble factor secreted from CD11c⁺ myeloid dendritic cells impairs MCMV^r replication in a reversible manner. LSEC were infected with MCMV^r at an MOI of 0.0001 in dodecuplicates and cultured afterwards with medium control or filtered SN from coculture of infected LSEC plus CD11c⁺ mDC. Wells were analyzed at multiple time points, and those with even one infected cell were classified as positive. At dpi 5, SN was removed in selected wells (+/−) and wells were further cultured with RPMI medium. (A) Representative EYFP fluorescence and bright-field microscopy of medium control or LSEC cultured in filtered SN from coculture 1 and 7 days after SN retraction. (B) Percent of wells showing any viral gene expression at the indicated time points (right panel). Group averages ± SEM from three independent experiments are shown. Supernatant was collected at indicated time points and titrated on MEF (left panel). To monitor for virus reactivation, only wells which showed no viral replication on day 5 were monitored.