FIG 6.

MHV3-induced breakdown of BMEC barrier does not depend on inflammatory factors. (A to H) bEnd.3 cells were infected with MHV3, MHV-A59, and 51.6-MHV3 at an MOI of 0.1, and mRNA expression (A, C, E, and G) and protein levels (B, D, F, and H) of TNF-α, IL-6, CCL2, and CXCL10 were evaluated by qRT-PCR and ELISAs, respectively, at 24 h p.i. All samples were run in duplicate. All experiments were conducted in triplicate. Results are representative of two independent experiments. Values that are significantly different are indicated by asterisks as follows: *, P < 0.05; **, P < 0.01; ***, P < 0.001.