FIG 3.

Relative concentrations of TYLCV based on the gene sequences of V1, V2, and C3 measured by using qPCR, compared to the expression levels of the β-actin housekeeping gene and in a control experiment. (A and B) Relative concentrations of TYLCV in B. tabaci adult females after an 8-h AAP on TYLCV-infected tomato and exposure to 0.5 mg a.i./liter of acetamiprid for 5 days (A) or imidacloprid for 3 days (B). (C) Relative concentrations of TYLCV measured by qPCR based on V1, V2, and C3 sequences in dissected abdomens from adults that had acquired the virus for 8 h and retained it for 7 days. (D) Relative concentrations of TYLCV, based on the three gene sequences, in adults that had acquired the virus for 8 h and retained it for 4 days under heat stress at 35°C. Data shown are the means ± standard errors of the means of data from three independent experiments.