FIG 4.

MST-312 reduces HSV-1 protein accumulation and cytopathic effects in hTERT-HME1 cells in a dose-dependent manner. hTERT-HME1 cells were infected with HSV-1 KOS1.1 (KOS), at an MOI of 10, in the presence MST-312 at the indicated concentrations or DMSO. At 18 hpi, Hoechst dye was added to the medium (10 μg/ml), and cells were photographed and harvested for immunoblot assays. (A) Cytopathic effect determined by observation by phase (Phase) and fluorescence (Hoechst) microscopy. (B) Immunoblot analysis of viral protein accumulation using antibodies specific for ICP4, ICP27, and VP22. Numbers below viral protein blots represent normalized protein band densities relative to the band density for KOS1.1-infected DMSO-treated control cells. − indicates density values that are below the threshold of detection.