FIG 6.

EGCG and delphinidin do not alter HCVcc integrity and do not aggregate HCVcc. (A) HCVcc was incubated with 50 μM EGCG, 50 μM delphinidin, 50 μM petunidin, DMSO, or 0.5% Triton X-100 in the presence of RNase A at 50 μg/ml. Treated particles were then separated on 10 to 40% iodixanol equilibrium gradients. Viral RNA quantity, infectious titer, and buoyant density were determined in each fraction. Data are the mean ± SEM of three experiments performed in triplicate. (B) After incubation with DMSO or 250 μM EGCG or delphinidin, HCVcc virions were ultracentrifuged in a 5 to 20% iodixanol velocity gradient. HCV RNA was quantified by qRT-PCR. Data are representative of three experiments.