FIG 5.

c-Myc degradation after MeV infection. (A) 293SLAM cells were infected with MeV for 24 h and then immunoblotted with anti-c-Myc, anti-MeV-N protein, and anti-GAPDH antibodies. (B) 293SLAM cells were transfected with a reporter plasmid, pGL3-basic or pGL3-Ebox, together with phRG-B as an internal reference. After 24 h, the cells were mock treated or infected with MeV for 24 h and then subjected to a dual-luciferase assay. The results are expressed as values relative to the value of the mock-treated control sample. (C) 293SLAM cells were infected with MeV for 24 h, treated with MG132 or the same volume of DMSO in fresh culture medium for the indicated times and then immunoblotted to detect c-Myc and MeV-N protein. (D) 293SLAM cells were treated with a DNA-PK inhibitor or the same volume of DMSO for 24 h and then immunoblotted to detect c-Myc.