FIG 1.

Alignment of pORF30 terminase homologs of human alpha- and betaherpesviruses. Clustal Omega was used to align the pORF30 terminase subunit primary amino acid sequences for 3 human alphaherpesviruses (HSV-1, HSV-2, and VZV) and 4 human betaherpesviruses (HCMV, HHV-6A, HHV-6B, and HHV-7). The human gammaherpesviruses HHV-4 and HHV-8 were not included. Red sequences indicate the 12 conserved regions previously identified in HCMV pUL56 by Champier et al. (15). Green sequences represent the 15 conserved regions identified using the current alignment and are in close agreement with the previous analysis by Champier et al. (15). A highly conserved stretch of 11 amino acids found in the C terminus of region IX is indicated with x's. The conserved putative zinc finger motif, C-X₂-C-X₂₂-C-X-H (arrows mark the conserved cysteine/histidine residues), was found in region IV of all nine human herpesviruses. *, identical residues; colons, highly conserved residues; periods, conserved residues. Identity/conservation is indicated for alignment of the 3 alpha- and 4 betaherpesvirus pORF30 homologs; however, only the VZV and HCMV sequences are shown.