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Systemic administration of rapamycin modulates the mammalian target of rapamycin signaling pathway and autophagy in a rat fracture model. Calluses from rats were collected at 2, 4 and 6 weeks post-fracture after treatment with rapamycin or the vehicle (n=12 per group). (A) Sections were analyzed using immunohistochemistry for phosphorylation of ribosomal protein S6. (B) Sections were analyzed by immunofluorescence for light chain 3-II (magnification, ×200).
