Figure 2.

Schematic for identification of protein interfaces in the Protein Data Bank (PDB) where a helix dimer contributes significantly to complex formation. Interfacial helices from the previously described HippDB dataset were culled to produce a set of structures for detailed analysis via stringent distance, orientation, and energetic criteria. On the basis of our evaluations, we classified the interactions among three classes (Cases 1–3) of helical tertiary structure-mediated PPIs. The energetic contribution of each interface helix dimer and individual residues was approximated using Rosetta. The complete dataset is hosted at www.nyu.edu/projects/arora/dippdb/cc.php.