Figure 2.

mRNA expression levels of Orai and Stim genes and subcellular localization of STIM1 in LS8 cells. Relative mRNA quantification of Orai (1 to 3; A) and Stim (1 and 2; B) in LS8 cells. Each experiment was repeated a minimum of 3 times. Hprt1 was used as the reference gene. (C) Confocal microscopy showing localization of STIM1 (red) and the endoplasmic reticulum marker calnexin (CNX; green) in LS8 cells. DAPI is shown as blue nuclear staining. Panels A and B are representative of a minimum of 3 independent experiments.