Figure 3.

Ca²⁺ entry is inhibited by CRAC (Ca²⁺ release-activated Ca²⁺) channel blockers in ameloblast-like LS8 cells. (A) Ca²⁺ measurements in LS8 cells. Representative Ca²⁺ traces showing the Fura-2 fluorescence ratios (340/380 nm) of Fura-2/AM-loaded control LS8 cells (white tracings) and cells pretreated with the CRAC channel inhibitors Synta-66 (3 µM, black), BTP-2 (300 nM, blue), and 2-APB (50µM, red). Where indicated, 1.25µM thapsigargin (TG) was added to the nominally Ca²⁺-free extracellular bath solution to measure the release of Ca²⁺ from endoplasmic reticulum stores followed by the addition of 2mM extracellular Ca²⁺ to induce store-operated Ca²⁺ entry (SOCE). Arithmetic means ± SEM of the peak (left) and slope (right) of the change in Fura-2 ratio following addition of TG (Ca²⁺ release; B) and readdition of 2mM Ca²⁺ (SOCE; C) of extracellular Ca²⁺ in LS8 cells. Untreated control (n = 6 independent experiments, white bars), Synta-66 pretreated (n = 6, black bars), BTP-2 pretreated (n = 6, blue bars), 2-APB pretreated (n = 6, red bars). ***P < 0.001. Analysis of variance.