Table 1.
Primer sequences for amplification of different vacuolar, papain-like cysteine proteases and redox-associated proteins; primers for γECS, hGS, GR MDAAR and DHAR were designed based on NCBI reference sequences
| Phytozome ID | Forward primer (5′ to 3′) | Reverse primer (3′ to 5′) |
|---|---|---|
| Glyma18g52780 (Actin 1) | TGTTCCCTGGTATTGCTGAC | AAGGTGCTAAGAGATGCCAAG |
| Glyma02g44460 (ElF 1-β) | GTGGTACGATGCTGTCTCTC | CCACTGAATCTTACCCCTTGAG |
| γECS (XM_006586262.1) | CTCAAACAGGGGAAGCAGAG | CACTTTGGCTGGAAACCAAT |
| hGS (NM_001250121.1) | AATTCTGGTCGTGGTTCAGG | TGAAATTGCTTGTCCATCCA |
| MDAR (XM_006599044.1) | GTGGTGGGAATTGGAATACG | TGCTTTGACTGGAAATGCTG |
| DHAR (NM_001250000.1) | GAGATTGCTTTGGGGCATTA | TTCCACTTTAGGACGCCAAC |
| Glyma06g41610 (Tx1) | CAGTGGATAAAATTGTGGGTGC | GGCAACTGTTCAATGTGTTCG |
| Glyma07g09240 (Prx1) | TGAAAGGAAAGGGTGTGGAC | GAGGGCATTGGTGTATTTGG |
| Glyma09g32540 (Prx2) | ACGTTCCTGGCTTCATTGAG | TTCTCTGGGAATGTTTTGGC |
| Glyma12g13920(Grx1) | TTCCTTATAGGTCATGGCAACC | GTGTGGGCTAATTGTGAAGTG |
| Glyma16g07870 (Grx2) | ACCTATTGCCCTTTCTGC | TCCGTCCACTCAACTAATGC |
| Glyma17g14680 (VPE1) | CTACGGAAACTACAGGCATC | GTTCTCCGTCGTCACATTAT |
| Glyma05g04230 (VPE2) | CACCATCCCTTGTAAATTGT | GGGGTTTCAGTGCATAATAA |
| Glyma14g10620 (VPE3) | GGTCGTGGATGTTGCTGAGG | ATCTGCTTGATGCCTGTAGTTTCC |
| Glyma04g04400 (CP1) | GATCTTTAATGGCCACGATCCTCAT | CAGCACCTTGAAAGGGGTAATCCT |
| Glyma17g05670 (CP2) | GCTTGTCACTGCTCATTTTCGC | TTTTCCGGTGTAGGGATATGC |
| Glyma10g35100 (CP3) | GAGGCCATGCCCTCATGT | TCACCTCTCTCCCCAGTGTAGG |
| Glyma14g40670 (CP4) | ATATGGAGCGTGTGACTCGG | GTAATATCCATTCTCTCCCCAGCTC |
| Glyma04g03090 (CP5) | AAGCTGTGGTGCATGTTGGG | AGTGGCGCTTGTCTTTGCAG |