Table 1.
List of 52 kiwifruit proteins identified by nanoLC-MS/MS
| Spot no.* | Protein name† | Accession no.‡ | SNP§ | Responsiveness to SNP C/U/D¶ | O3** | Responsiveness to O3 C/U/D†† | SNP + O3‡‡ | Responsiveness to SNP + O3 C/U/D§§ | Sub-cellular localization¶¶ | Functional categories*** |
|---|---|---|---|---|---|---|---|---|---|---|
| 1204 | Kiwellin | gi|85701136 | N | C | N | C | Y | D | Extracellular | 11·02-Disease/defence/Defence-related |
| 1705 | HSP70 | gi|255573627 | Y | U | N | C | N | C | Endoplasmic reticulum | 06·01-Protein destination and storage/Folding and stability |
| 1810 | HSP0 luminal binding | gi|168057678 | N | C | N | C | Y | U | Endoplasmic reticulum | 06·01-Protein destination and storage/Folding and stability |
| 2607 | Glutamate decarboxylase | gi|383745688 | Y | U | N | C | N | C | Chloroplast | 01·01-Metabolism/Amino acid |
| 2711 | Chaperonin CPN60 | gi|356534856 | Y | U | N | C | N | C | Mitochondrion | 06·01-Protein destination and storage/Folding and stability |
| 2716 | Leucine aminopeptidase | Achn369241 | N | C | Y | U | Y | U | Chloroplast | 06·13-Protein destination and storage/Proteolysis |
| 3116 | Kiwellin | gi|85701136 | N | C | Y | U | N | C | Extracellular | 11·02-Disease/defence/Defence-related |
| 3203 | Kiwellin | gi|85701136 | Y | D | N | C | N | C | Extracellular | 11·02-Disease/defence/Defence-related |
| 3207 | Aldehyde dehydrogenase | gi|57471048 | Y | D | N | C | Y | D | Mitochondrion | 11·06-Disease/Defence/Detoxification |
| 3304 | Glutelin type-A | gi|195225381 | N | C | Y | D | Y | D | Cytoplasm | 06·20-Protein destination and storage/Storage proteins |
| 3421 | Kiwellin | gi|85701136 | Y | D | Y | D | Y | D | Extracellular | 11·02-Disease/defence/Defence-related |
| 3423 | Polyphenoloxidase | gi|195241520 | Y | D | Y | D | N | C | Chloroplast | 20·1-Secondary metabolism/Phenylpropanoids/Phenolics |
| 3507 | Enolase | Achn246351 | N | C | N | C | Y | U | Plasma membrane | 02·01-Energy/Glycolysis |
| 3511 | Polygalacturonase | gi|195226843 | N | C | Y | D | Y | D | Cell Wall | 09·01-Cell structure/Cell wall |
| 3512 | Enolase | gi|14423687 | N | C | Y | D | N | C | Cytoplasm | 02·01-Energy/Glycolysis |
| 3715 | ATP-citrate synthase | Achn228301 | N | C | Y | U | N | C | Cytosol | 01·06-Metabolism/Lipid and sterol |
| 3716 | F1-ATPase alpha subunit | gi|404481694 | N | C | N | C | Y | U | Mitochondrion | 07·22-Transporters/Transport ATPases |
| 3717 | Glutelin type-A | gi|195225381 | N | C | Y | D | Y | D | Cytoplasm | 06·20-Protein destination and storage/Storage proteins |
| 3718 | Sucrose synthase | gi|398363593 | N | C | Y | D | Y | D | Cytoplasm | 01·05-Metabolism/Sugars and polysaccharides |
| 3719 | Viral A-type inclusion protein repeat containing protein expressed | gi|356510118 | N | C | Y | D | Y | D | Nucleus | 10·04-Signal transduction |
| 3721 | Viral A-type inclusion protein repeat containing protein expressed | gi|356510118 | N | C | Y | D | Y | D | Nucleus | 10·04-Signal transduction |
| 3727 | HSP70 | gi|190442576 | N | C | N | C | Y | U | Cell wall | 06·01-Protein destination and storage/Folding and stability |
| 4003 | Bet v 1 related allergen | gi|281552896 | N | C | N | C | Y | U | Cytoplasm | 11·02-Disease/defence/Defence-related |
| 4408 | Malate dehydrogenase | Achn221601 | Y | D | N | C | N | C | Mitochondrion | 02·10-Energy/TCA pathway |
| 4707 | Phosphoenolpyruvate carboxykinase | gi|195203610 | N | C | Y | D | Y | D | Cytoplasm | 02·02-Energy/Gluconeogenesis |
| 4710 | Pyruvate decarboxylase | gi|51587336 | N | C | Y | D | Y | D | Chloroplast | 11·05-Disease/Defence/Stress responses |
| 5710 | Phosphoenolpyruvate carboxykinase | Achn041831 | N | C | N | C | Y | D | Cytoplasm | 02·02-Energy/Gluconeogenesis |
| 5712 | Non-identified | N | C | N | C | Y | D | 13-Unclassified | ||
| 6207 | Kiwellin | gi|587936047 | N | C | Y | U | N | C | Extracellular | 11·02-Disease/defence/Defence-related |
| 6209 | Kiwellin | gi|85701136 | N | C | Y | U | N | C | Extracellular | 11·02-Disease/defence/Defence-related |
| 6309 | Fructose-bisphosphate aldolase | Achn044851 | N | C | N | C | Y | D | Cytoplasm | 02·01-Energy/Glycolysis |
| 6411 | Triosphosphate isomerase | gi|195201084 | N | C | N | C | Y | U | Cytosol | 02·01-Energy/Glycolysis |
| 6509 | Non-identified | N | C | Y | D | N | C | 13-Unclassified | ||
| 6510 | Non-identified | N | C | N | C | Y | D | 13-Unclassified | ||
| 7417 | Fructose-bisphosphate aldolase | gi|587847389 | N | C | N | C | Y | D | Cytoplasm | 02·01-Energy/Glycolysis |
| 7605 | Non-identified | N | C | N | C | Y | D | 13-Unclassified | ||
| 7714 | NADP-dependent malic enzyme | Achn312431 | N | C | N | C | Y | D | Chloroplast | 02·10-Energy/TCA pathway |
| 7802 | Remorin | Achn227731 | N | C | N | C | Y | D | Nucleus | 10·04-Signal transduction |
| 7805 | NADP-dependent malic enzyme | Achn312431 | N | C | N | C | Y | D | Chloroplast | 02·10-Energy/TCA pathway |
| 7806 | Remorin | Achn227731 | N | C | N | C | Y | D | Nucleus | 10·04-Signal transduction |
| 7807 | NADP-dependent malic enzyme | Achn312431 | Y | U | N | C | Y | D | Chloroplast | 02·10-Energy/TCA pathway |
| 7901 | Beta-d-galactosidase | gi|318136780 | N | C | Y | D | Y | D | Cell wall | 09·01-Cell structure/Cell wall |
| 7903 | Beta-d-galactosidase | gi|318136780 | N | C | N | C | Y | D | Cell wall | 09·01-Cell structure/Cell wall |
| 7907 | Beta-d-galactosidase | gi|318136780 | N | C | Y | D | Y | D | Cell wall | 09·01-Cell structure/Cell wall |
| 8208 | Thaumatin | gi|146737976 | Y | U | N | C | N | C | Extracellular | 11·02-Disease/defence/Defence-related |
| 8804 | Beta-d-galactosidase | gi|318136780 | N | C | Y | D | Y | D | Cell wall | 09·01-Cell structure/Cell wall |
| 9310 | Annexin | Achn387941 | N | C | N | C | Y | D | Cytoplasm | 11·05-Disease/Defence/Stress responses |
| 9316 | Annexin | Achn387941 | N | C | N | C | Y | D | Cytoplasm | 11·05-Disease/Defence/Stress responses |
| 9319 | Endochitinase | gi|195305994 | N | C | N | C | Y | D | Cell Wall | 11·02-Disease/defence/Defence-related |
| 9411 | Endochitinase | gi|195199630 | N | C | Y | D | Y | D | Extracellular | 11·02-Disease/defence/Defence-related |
| 9421 | GAPDHC | gi|166864050 | N | C | N | C | Y | D | Cytoplasm | 02·01-Energy/Glycolysis |
| 9506 | Pectinacetylesterase precursor | Achn130871 | N | C | Y | D | Y | D | Cell Wall | 09·01-Cell structure/Cell wall |
*Spot no, spot label on the reference gel maps presented in Supplementary Data Fig. S3.
†Protein name, identified peptide names.
‡Accession no., accession number in NCBI or the Kiwifruit Genome database.
§SNP, protein whose accumulation level varied under SNP treatment Y, yes; N, no.
¶Responsiveness to SNP, pattern of accumulation; C, protein whose accumulation level was constant; U, upregulated protein; D, downregulated protein.
**O3, protein whose accumulation level varied under O3 treatment Y, yes; N, no.
††Responsiveness to O3 pattern of accumulation; C, protein whose accumulation level was constant; U, upegulated protein; D, downregulated protein.
‡‡SNP + O3, protein whose accumulation level varied under SNP + O3 treatment Y, yes; N, no.
§§Responsiveness to SNP + O3 pattern of accumulation; C, protein whose accumulation level was constant; U, upregulated protein; D, downregulated protein.
¶¶Sub-cellular localization, sub-cellular localization was assigned based on database searches.
***Functional category, proteins ontologically classified into functional categories proposed by Bevan et al. (1998).