Fig. 3.

Inhibitors of NTR activity interfere with auxin response. Arabidopsis thaliana transgenic plants expressing the BA3 : : GUS and DR5 : : GUS genes were grown in ATS-agar for 4 d. Plants were incubated in ATS solution containing 0·5 µm NAA or not (−) and with or without (control) the NTR inhibitors either ANF or DNCB, at the indicated concentrations, for 18 h. Plants were incubated at 37 °C in the presence of the substrate X-Glu for 2 h. Pictures were taken under a magnifier. Scale bars = 2 mm. (B) Densitometric analysis of roots treated with NAA with or without the inhibitors ANF and DNCB using Image J software. Bars denote standard error of at least two independent experiments (n = 5).