Fig. 5.

The arabidopsis mutant ntra ntrb has increased levels of S-nitrosylated proteins. Arabidopsis root extracts of wild-type or ntra ntrb mutants were in vitro S-nitrosylated with 500 µm CysNO for 30 min. (A) S-nitrosylated proteins were detected by the biotin switch method using 10 mm ascorbate (ASC) as a specific reductant of S-nitrosothiols. (B) Total oxidized Cys were detected by the biotin switch method using 20 mm dithiothreitol (DTT). Biotinylated Cys were detected by an anti-biotin antibody.