Figure 1.

Reduced TBX21 and IFNG mRNA expression in the tumoral region of NSCLC. (A and B) qPCR-based expression analysis of TBX21 mRNA in lung tissue samples from the tumoral, peri-tumoral and control region of patients with adenocarcinoma (ADC) or squamous cell carcinoma (SCC; ADC: N_Control=16, N_Peri-tumoral=15, N_Tumoral=16; SCC: N_Control=17, N_Peri-tumoral=17, N_Tumoral=17). (C and D) Correlation between the tumour diameter and the TBX21 mRNA expression in the control area of patients with ADC and SCC. (E and F) qPCR-based expression analysis of IFNG mRNA in lung tissue samples from the tumoral, peri-tumoral and control region of patients with ADC or SCC (ADC: N_Control=14, N_Peri-tumoral=13, N_Tumoral=14; SCC: N_Control=16, N_Peri-tumoral=16, N_Tumoral=17). (G) Correlation between TBX21 and IFNG mRNA levels in the control as well as in the peri-tumoral and the tumoral area of patients with non-small-cell lung cancer (NSCLC). (H) Flow cytometry analysis of T-bet⁺CD4⁺ and T-bet⁺CD8⁺ T cells gated on lymphocytes from the control, the peri-tumoral and the tumoral lung region of one representative patient with NSCLC. Data are shown as mean values±s.e.m. using Student's t-test *P=0.05; **P=0.01; ***P=0.001.