Figure 7.

Increased killing capability of CD8⁺ T cells from the control area. CD8⁺ T cells were isolated from the tumoral, peri-tumoral and control region of tissue samples from the lung of lung cancer patients. CD8⁺ cells were co-cultured with total cells from the tumoral region of the respective patient (tumour cells). Apoptosis and necrosis levels were evaluated by flow cytometry. Cells were double-stained with antibodies against Annexin V and PI. (A) Tumour cell gate and a representative flow cytometry plot of Annexin V versus PI staining is depicted. (B–D) Bar graphs show the mean percentages of Annexin V or PI single positive as well as Annexin V and PI double positive cells. Data are shown as mean values±s.e.m. using Student's t-test *P=0.05.