Table 1. The mean cell density of different T-cell populations per region of interest.

	Alla	Tumour	Stroma
CD3
HPV positive∼	7.9 × 103	3.5 × 103	4.2 × 103
HPV negative	3.2 × 103	1.5 × 103	1.6 × 103
P-value	<0.0001	<0.0001	0.1
CD3+CD8+
HPV positive	1.8 × 103	0.5 × 103	1.3 × 103
HPV negative	0.4 × 103	0.25 × 103	0.14 × 103
P-value	<0.0001	<0.0001	<0.0001
CD3+CD4+
HPV positive	5.1 × 103	2.3 × 103	2.4 × 103
HPV negative	1.6 × 103	0.8 × 103	0.9 × 103
P-value	<0.0001	<0.0001	<0.001
CD4+FoxP3+
HPV positive	0.1 × 103	0.006 × 103	0.005 × 103
HPV negative	0.09 × 103	0.003 × 103	0.004 × 103
P-value	0.1	0.2	0.09

Abbreviartions: HPV=human papillomavirus; OPSCC=oropharyngeal squamous cell carcinoma; ROI=region of interest.

^aMean of 60 ROIs, each 3.5 × 10⁵ nm² (30 in stroma and 30 in tumour areas), ∼74 HPV-positive and 65 HPV-negative OPSCC.