Figure 3.

(5Z)-7-oxozeaenol sensitises tumour cells to radiation. (A and B) FLO-1 and KYAE-1 cells were treated with (5Z)-7-oxozeaenol or dimethyl sulfoxide (DMSO) as control, and then irradiated as indicated. Western blot analysis of Baculoviral inhibitor of apoptosis (IAP) repeat containing (BIRC)3, cleaved poly (ADP-ribose) polymerase 1 (PARP1) and cleaved caspase 3 levels was performed after 48 h form the treatment. γ-Tubulin was detected as a loading control. (C) The extent of apoptosis was determined after 48 h of treatment by Annexin V and propidium iodide staining and flow cytometric analysis. Data shown are representative of three independent experiments.