Figure 3.
Immunohistochemical analysis of HLA-I HC and tapasin expression in tumour sections corresponding to GCLs. Paraffin-embedded tissue sections were deparaffinised, rehydrated and stained using the MACH 2 system (Biocare Medical) and (A) HLA-I or (B) tapasin antibody. Positive Pixel score (PpS) was calculated as described in Thuring et al, 2014. (C) Correlation analysis with calculation of Pearson's correlation coefficient for expression of tapasin and HLA-I HC in immunohistochemical analysis of tumour sections (P<0.0001). (D) Correlation analysis with calculation of Pearson's correlation coefficient for expression of tapasin in tumour tissue plotted against days of survival for patients corresponding to tumour tissues (P<0.01). Presented here is the data from the 11 GBMs that we have analysed in this study together with the 12 tumours analysed in a previous paper by our group (Thuring et al, 2014).