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. 2015 Jan-Feb;10(1):75–83.

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Copyright: © Research in Pharmaceutical Sciences

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Fig. 3 — SDS–PAGE of primary isolation of ScFv expressed in E. coli strain BL21(DE3) pLysS in the form of inclusion bodies. Lane 1; molecular weight marker, lane 2; bacterial culture before induction, lane 3; bacterial culture after induction, lane 4; inclusion bodies fraction after centrifugation, lane 5; inclusion bodies fraction after Triton X-100 wash, lane 6; inclusion bodies fraction solubilized with GndHCl, lane 7; inclusion bodies fraction solubilized with Urea, lane 8; purified ScFv with Ni-NTA column.