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. Author manuscript; available in PMC: 2015 Sep 22.
Published in final edited form as: Mol Cell. 2014 Sep 18;56(2):193–204. doi: 10.1016/j.molcel.2014.08.020

Figure 3. EVs Rapidly Adhere to and Are Incorporated into Target Cells via Plasma Membrane.

Figure 3

(A) CD63-RFP EV uptake in fEGFP target cells (in green) as early as 2 hr after transfer. EVs are in red under confocal and magenta under super-resolution STED microscopy. Scale bars: top left, 5 μm; top right, 2 μm; bottom, 1 μm.

(B) FCM analysis of EGFP internalization by target cells treated with increasing quantities of EVs. Data are represented as mean relative fluorescence intensity ± SEM from n = 3 independent experiments. A representative confocal image of a NIH 3T3 cell (red) exposed to fEGFP EVs (green) is shown. The lower panel is a Z stack of n = 5 optical slices taken at 1 μm intervals. Scale bar, 10 μm.

Nuclei in (A) and (B) were stained with DAPI (blue). See also Movie S1.