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. Author manuscript; available in PMC: 2016 Sep 17.
Published in final edited form as: Chem Biol. 2015 Sep 10;22(9):1185–1196. doi: 10.1016/j.chembiol.2015.08.007

Figure 4. Cell cycle-dependent PLK1 expression requires KDM4 activity.

Figure 4

(A) Western blot of indicated proteins from thymidine-released LNCaP cells. (B) Relative KDM4 demethylase activity at various time points of double thymidine released LNCaP cells. The activities were expressed relative to asynchronized (asy) cells. (C) Relative occupancy of H3K9me3, KDM4B, and BMYB on the PLK1 promoter at various time points of double thymidine-released LNCaP cells. The amounts of immunoprecipitated complex were normalized against DNA input and expressed relative to that from asynchronized cells. (D) Relative fold change of mRNA of the indicated genes from LNCaP cells described in (B) in the presence or absence of compound B3. mRNAs were expressed relative to the 0 h time point.