Fig. 2.

ApoJ attenuates the increased ROS production via PI3K-Akt pathway induced by AngII. a Western blot analysis of Nox2/gp91phox expression in NRVCs. Nox2/gp91phox expression was enhanced by AngII in control adenovirus-infected cells, while ApoJ-transduced cells inhibited its expression (n = 3/group). b LY294002 was used to pretreat ApoJ-transduced NRVCs for 1 h, and then the control and ApoJ-transduced NRVCs were incubated with AngII for 24 h. Nox2/gp91phox expression was determined by Western blot (n = 3/group). c AngII increased pAkt expression in control infected NRVCs, and ApoJ further augmented its expression. d Cell apoptosis caused by AngII increased in ApoJ-overexpressing cells treated with LY294002. ^* P < 0.05 versus control Ad/PBS group, ^** P < 0.01 versus control Ad/PBS group; ^# P < 0.05 versus control Ad/AngII group, ^## P < 0.01 versus control Ad/AngII group,^$ P < 0.05 versus ApoJ group