Table 1.

Chloroplast SSRs developed in this study, showing region, primers, motif, melting temperature, suggested pool and dye color for multiplexing, and GenBank accession number for sequences from Artocarpus camansi (NTBG 960,576.001).

Locus	Region	Primer sequences (5′–3′)	Repeat motif	Ta (°C)a	Pool/Dye	GenBank accession no.
AALTCP01	ndhA	F: TTGGGGCTTTACGTTGGTAG	(T)9, (C)7(T)7	60.0	1/D4	KR185519
		R: CGTTTCTATTCTTCTTTCTCTTTCTG		58.5
AALTCP02	ndhA	F: CAGAAAGAGAAAGAAGAATAGAAACG	(A)10, (TA)7	58.5	2/D4	KR185520
		R: AAACTCGTCCTTTTCACTTACGA		59.4
AALTCP03	petB	F: ACCTCGTGGCCGGACTTT	(T)14	63.0	4/D4	KR185521
		R: TCCTTGAGTAAGAACCGTTGG		59.2
AALTCP04	petB–petD	F: TCACTTGGGGTAGGAACAATAG	(TA)6, 22-bp indel	58.1	1/D3	KR185522
		R: TTCTGCATAGCCCAATCAAT		58.1
AALTCP05	psbE–petL	F: TTCCAAGGATAGGGCTTTGT	(A)11	58.7	4/D3	KR185523
		R: TTTTATTGTATCGCCGAATCC		59.0
AALTCP06	rpl16	F: TGAATCATCCACCTTACCTTACA	(T)7A(T)9	58.5	1/D3	KR185524
		R: CATCGTTTCGCATTATCTGG		59.1
AALTCP07	rps8–rpl14	F: TTTTTATTCATGTCAGCATTTCG	(T)10	59.1	5/D4	KR185525
		R: AGGAAATTGTTTGTGTCTCACG		59.1
AALTCP08	rpl14–rp116	F: TCAAATGGGTTTGAGGTTGA	(A)11, (T)9	59.0	3/D4	KR185526
		R: AGCGGTATCCAAAATGCCTA		59.6
AALTCP09	trnS–trnG	F: TCCGACGCTTTAGTCCACTC	(T)13, 5-bp indel	60.4	2/D3	KR185527
		R: GCCAAGCCGTGTAAAGAAAA		60.2
AALTCP10	trnS–trnG	F: GGGCCTCTTTTGTTCTAACG	(T)9, (A)9	58.8	3/D2	KR185528
		R: TGTTCAAAAATTCATAGTTCTTTTGTT		58.7
AALTCP11	rps16	F: GCCGTACGAGGAGAAAACTTC	(G)5A(G)9	60.3	4/D2	KR185529
		R: GCGCCCTTTTCAAGGAAATA		61.4
AALTCP12	rps16	F: GCTCTTCGGAAAGTGGGTTT	(AT)6	60.6	5/D4	KR185530
		R: TCATTCACACCTTAACGCTCT		57.5
AALTCP13	rps16	F: GAAAGTGCTTTTGGCTCGAC	(T)12(G)10	60.0	3/D3	KR185531
		R: AGATTCTTGCCTCCGAAAAA		58.9
AALTCP14	trnT–trnE	F: CGGATTTGAACCGATGACTT	(TA)5	59.9	2/D2	KR185532
		R: TCGTTCCTGAGTGAACCACTA		58.3
AALTCP15	trnT–trnE	F: TGGTTCACTCAGGAACGATAAA	(A)8	59.6	1/D2	KR185533
		R: TGGATCTAGGTTGAATTGGTAGG		59.4

Note: T_a = annealing temperature.

^aAll primers amplified with an annealing temperature of 59.8°C (step 1) and 55°C (step 2).