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. 2015 May 6;3(3):196–208. doi: 10.1002/iid3.59

Figure 4.

Figure 4

IL-33-induced expression of MHC class II is partly reversible. (a) The schedule of experiments. IL-33 was added to culture medium for 5 days, and later removed. BMMCs were analyzed by FACS and real-time PCR on the day indicated. (b) Flow cytometric analysis of the surface expression level of MHC class II using FITC labeled I-Ad antibody. Shaded histograms are the isotope control. Immediately after removal of IL-33 on day 0 (blue line), day 1; absence of IL-33 for 1 day (green line), day 3; absence of IL-33 for 3 days (pink line) and day 5; absence of IL-33 for 5 days (sky blue line). A representative result of three independent experiments is shown. (c,d) Kinetics of reversible of I-Ad mRNA measured by real-time PCR. mRNA expression of CIITA types III and IV was measured in the absence of IL-33 for 1 and 5 days. A representative result of three independent experiments is shown. Data represent the mean and SEM [(c,d) n = 3)], and differences between BMMCs and IL-33-treated BMMCs are indicated. *P < 0.05, t-test.