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. 2014 Oct 29;4:e967616. doi: 10.4161/21592527.2014.967616

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© 2014 The Author(s). Published with license by Taylor & Francis Group, LLC

© Anna Burmester, Bérengère Luthringer, Regine Willumeit, and Frank Feyerabend

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 5. — Qualitative gene expression. Comparison of gene expression of various genes involved in bone formation from human osteoblasts (OB) and osteosarcoma cell lines MG63, SaoS2 and U2OS under cell culture conditions (A) and after incubation with 25 mM MgCl₂ (B). The sizes of the DNA ladder are indicated in (C). GAPDH: glyceraldehyde 3-phosphate dehydrogenase; HPSE: Heparanase; ALP: alkalinephosphatase; RANKL: RANK ligand; BSP: bone sialoprotein; Cbfa1: runt-related transcription factor 2; OC: osteocalcin, OPN: osteopontin; OPG: osteoprotegerin; Col: Collagen.