Table 3.
Reverse transcription-PCR (RT-PCR) primer sequences
| Gene | abbreviation | RT-PCR-Primer (5’-3’) | Ta [°C] | product size |
|---|---|---|---|---|
| Glyceraldehyde 3-phosphate dehydrogenase40 | GAPDH | ACCACAGTCCATGCCATCAC | 55 | 452 |
| TTCACCACCCTGTTGCTGTA | ||||
| Heparanase41 | HPSE | TGGACCTGGACTTCTTCACC | 53 | 216 |
| TTGATTCCTTCTTGGGATCG | ||||
| Alkaline phosphatase40 | ALP | ACGTGGCTAAGAATGTCATC | 53 | 475 |
| CTGGTAGGCGATGTCCTTA | ||||
| Receptor Activator of NF-κB Ligand42 | RANKL | CAGGAGACCTAGCTACAGA | 49 | 418 |
| CAAGGTCAAGAGCATGGA | ||||
| Bone sialoprotein43 | BSP | AAGCAATCACCAAAATGAAGACT | 55 | 188 |
| TGGAAATCGTTTTAAATGAGGATA | ||||
| Runt-related transcription factor 244 | Cbfa1 | CCCCACGACAACCGCACC | 55 | 289 |
| CACTCCGGCCCACAAATCTC | ||||
| Osteocalcin40 | OC | CATGAGAGCCCTCACA | 48 | 315 |
| AGAGCGACACCCTAGAC | ||||
| Osteopontin 40 | OPN | CCAAGTAAGTCCAACGAAAG | 55 | 347 |
| GGTGATGTCCTCGTCTGTA | ||||
| Osteoprotegerin42 | OPG | AGACTTTCCAGCTGCTGA | 49 | 469 |
| GGATCTCGCCAATTGTGA | ||||
| Collagen type 1 | Col1 | AAAGGCAATGCTCAAACACC | 49 | 159 |
| TCAAAAACGAAGGGGAGATG | ||||
| Collagen type 245 | Col2 | GTGGAGCAGCAAGAGCAAGGA | 61 | 334 |
| CTTGCCCCACTTACCAGTGTG | ||||
| Collagen type 10 | Col10 | TCCAAAAGGTGATCCTGGAG | 55 | 181 |
| CCCTTTAGACCCAGGGAATC |