Fig. 3. Resveratrol modulates DC-mediated naïve CD 8+ T-cell proliferation. Immature DCs, OVA-pulsed DCs, OVA-pulsed IFN-γ-treated DCs, OVA-pulsed IFN-γ + resveratrol-treated DCs, or OVA-pulsed IFN-γ + resveratrol + GSK-3 inhibitor-treated DCs were cultured with CFSE-labeled splenocytes from OT-1 T-cell receptor transgenic mice (1 × 106 per well) for 96 h. The cells were harvested after 4 days, stained with Cy5-labeled anti-CD8 monoclonal Ab, and analyzed by flow cytometry. Histograms showing CD8+ T-cell proliferation as assessed by flow cytometry. Results are representative of three independent experiments.
