Fig. 1.

LDHA expression levels of melanoma brain metastases in mice displayed a biphasic pattern over time. (A) LDHA immunohistochemistry of brain metastases of increasing size (diameters provided) over 4 wk post intracardiac injection (n = 3 per wk). Inset displays representative staining pattern within the largest metastasis. (B) LDHA score vs brain metastasis size (n = 59; Spearman correlation). No metastases got an LDHA score 1–3. (C) LDHA scores of small (≤305 μm; n = 30) vs large (>305 μm; n = 29) brain metastases (median split; Pearson chi-square test, P = .038).