Fig. 1. H₂O₂-induced DSBs activate DNA damage repair in MOVAS. (A) Representative images of alkaline comet assays at 20, 60, and 120 min following H₂O₂ treatment. Comet tails were found in H₂O₂ treated cells. (B) Quantification of DNA damage by tail moment of comet assay treated with or without H₂O₂. Tail moments were measured from three independent experiments with at least 30 cells at single time points per sample. (C) Cells were treated with various concentrations of H₂O₂, and visualized by immunostaining with anti-γH2AX (red) following 1 h H₂O₂ treatment. Nuclei were stained with Hoechst 33258 (blue). (D) The number of γH2AX foci in cells was counted (n = 15). The error bars represent the standard deviation, *P ＜ 0.05.