Fig. 4. PARG inhibitor reduced oxidative DNA damage level and cell death. (A) Following PARG treatment, PAR synthesis in MOVAS was examined by dot blot. (B, C) MOVAS were pre-treated with or without PARG inhibitor followed by H₂O₂. DNA breaks were examined at 20 min following H₂O₂ treatment by alkaline comet assays. Tail moment was measured. (D, E) MOVAS were pre-treated with or without PARG inhibitor. H₂O₂-induced DSBs were examined and the foci of γH2AX in each cell were counted (n = 15). (F) MOVAS were pre-treated with or without PARG inhibitor and then exposed to 100 ㎛ H₂O₂. Cell viability was evaluated using an MTT assay. The error bars represent the standard deviation, *P ＜ 0.05.