Fig. 1. Upregulation of FZD6 in CRC patients. (A, B) Analysis of the Gene Expression Omnibus (GEO) database (accession numbers, GSE37364 and GSE21510) showed that FZD6 was significantly increased in CRC patients, versus the control group. P values were calculated using the by Mann-Whitney test. ***P ＜ 0.001. (C) The relative expression of FZD6 in the CRC tissues and adjacent non-tumor tissues of eight CRC patients. QRT-PCR analysis revealed that expression of FZD6 mRNA was increased in CRC tissues. The data was normalized against GAPDH mRNA expression. (D, E) Overexpression of FZD6 detected by immunohistochemistry in CRC patients compared to matching non-tumor samples. Scale bar = 200 μm.