Fig. 2. WNT4 expression was not altered in CRC patients. (A, B) Analysis of the Gene Expression Omnibus (GEO) database (accession numbers, GSE37364 and GSE21510) indicated that WNT4 expression showed no significant difference between CRC patients and control groups. P values were calculated using the by MannWhitney test. NS = not significant. (C) Relative expression of WNT4 in eight pairs CRC patients and their adjacent non-tumor tissues. QRT-PCR analysis also revealed that CRC tissues had no consistent expression pattern of WNT4 versus the control groups. Data were normalized against GAPDH mRNA expression.