Fig. 4. Inverse correlation between miR-199a-5p and FZD6 in CRC cells and tissues. (A) Relative expression of FZD6 mRNA in various miR-199a-5p-transfected CRC cells. All CRC cells showed reduced FZD6 expression with overexpression of miR-199a-5p. Data were normalized against GAPDH mRNA expression. Results are the average of three independent experiments conducted in duplicate. *P ＜ 0.05, **P ＜ 0.01, ***P ＜ 0.001. NS = not significant. (B) The relative expression of miR-199a-5p in eight pairs CRC patients and their adjacent samples. qRT-PCR analysis revealed that expression of miR-199a-5p was frequently decreased in CRC tissues. Data were normalized against GAPDH mRNA expression.