Fig. 6.

TFP5 rescues glucotoxic neuronal cell death. A) Primary cortical neurons, pre-treated with or without TFP5 or scrambled peptide, were incubated in low (LG) and high glucose (HG) for 8 h then prepared for immunocytochemistry. MAP-2 antibody was used as a neuronal marker; Texas red was used as the secondary antibody. Pretreatment with TFP5 protected MAP-2–positive neurons against the HG-induced cell loss (Bar = 100 µm). B) Quantification of the results. Cell counts were performed as follows: 10 independent fields were analyzed using NIH Image J software. The bar graph shows the number of MAP-2 labeled neurons as mean ± S.E.M. from four separate experiments (**p < 0.01).