Table 1. Differentially expressed proteins.
| Functional annotation a | Identified proteins b , c | Accession Number | NSAF SSD d | NSAF NSD e | P-value | Fold change |
|---|---|---|---|---|---|---|
| SSD proteins | ||||||
| Amino acid transport and metabolism | Glutamate dehydrogenase mitochondrial | EgrG_000604400.1 | 0,00218163 | 0 | - | - |
| 2 amino 3 ketobutyrate coenzyme A ligase | EgrG_000107200.1 | 0,00500288 | 0 | - | - | |
| Cytoskeleton | Myosin regulatory light chain A, smooth adductor muscle | gi|576695936 | 0,14420750 | 0,11952750 | 0,0027 | 1,2 |
| Actin cytoplasmic type 5 | EgrG_000190400.1 | 0,01251020 | 0,00709483 | 0,034 | 1,8 | |
| Myosin heavy chain, striated muscle | gi|576698220 | 0,02525800 | 0,01340450 | 0,0017 | 1,9 | |
| Dynein light chain 1, cytoplasmic | gi|576696895 | 0,13540500 | 0,06898675 | 0,0043 | 2,0 | |
| Myosin regulatory light chain 2 smooth muscle | EgrG_000041600.1 | 0,02763825 | 0,01373125 | 0,049 | 2,0 | |
| Actin cytoplasmic A3 | EgrG_000406900.1 | 0,01701225 | 0,00795388 | 0,0055 | 2,1 | |
| Energy production and conversion | ATP synthase subunit beta mitochondrial | EgrG_000752000.1 | 0,00210028 | 0 | - | - |
| NADP dependent malic enzyme | EgrG_001145700.1 | 0,00237255 | 0 | - | - | |
| Pyruvate dehydrogenase E1 component subunit | EgrG_000590700.1 | 0,00304668 | 0 | - | - | |
| NADH cytochrome b5 reductase 3 | EgrG_000865900.1 | 0,00339048 | 0 | - | - | |
| Aconitate hydratase mitochondrial | EgrG_000158240.1 | 0,00365168 | 0 | - | - | |
| Succinate dehydrogenase ubiquinone | EgrG_000422600.1 | 0,00774378 | 0 | - | - | |
| Pyruvate dehydrogenase | EgrG_000956200.1 | 0,00862753 | 0 | - | - | |
| Cytosolic malate dehydrogenase | EgrG_000417100.1 | 0,05691600 | 0,03523775 | 0,036 | 1,6 | |
| Succinate dehydrogenase (ubiquinone) iron sulfur | EgrG_000416100.1 | 0,04523500 | 0,02794375 | 0,00045 | 1,6 | |
| Phosphoenolpyruvate carboxykinase [GTP] | gi|576694081 | 0,07943825 | 0,04717400 | < 0,00010 | 1,7 | |
| Citrate synthase | EgrG_001028500.1 | 0,02500050 | 0,01324200 | 0,00059 | 1,9 | |
| Function unknown | Hypothetical protein | gi|576695609 | 0,00378138 | 0 | - | - |
| RNA binding protein EWS | EgrG_001020700.1 | 0,01534125 | 0,00991710 | 0,011 | 1,5 | |
| Expressed conserved protein | EgrG_000696700.1 | 0,00851018 | 0,00211125 | 0,015 | 4,0 | |
| Hypothetical protein | gi|576692667 | 0,01587075 | 0,00369325 | 0,023 | 4,3 | |
| Intracellular trafficking, secretion, and vesicular transport | Charged multivesicular body protein | gi|576693582 | 0,01026600 | 0 | - | - |
| Charged multivesicular body protein 4B | EgrG_001133200.1 | 0,02666625 | 0,01683500 | 0,041 | 1,6 | |
| Clathrin light chain | EgrG_000925600.1 | 0,01228048 | 0,00764348 | 0,025 | 1,6 | |
| Charged multivesicular body protein 5 | EgrG_001063800.1 | 0,01977925 | 0,00958983 | 0,011 | 2,1 | |
| Charged multivesicular body protein 3 | EgrG_000238400.1 | 0,02452800 | 0,00300075 | 0,0042 | 8,2 | |
| Nucleotide transport and metabolism | Nucleoside diphosphate kinase A 2 | gi|576695995 | 0,00894200 | 0 | - | - |
| Dihydropyrimidinase | EgrG_000953400.1 | 0,01286375 | 0,00754355 | 0,005 | 1,7 | |
| Post-translational modification, protein turnover, and chaperones | Heat shock 70 kDa protein 4 | EgrG_000938600.1 | 0,00130175 | 0 | - | - |
| 26S proteasome regulatory subunit T3 | EgrG_000223000.1 | 0,00212315 | 0 | - | - | |
| T-complex protein 1 subunit epsilon | gi|576697091 | 0,00235945 | 0 | - | - | |
| Calreticulin | gi|576693417 | 0,00287313 | 0 | - | - | |
| Activator of 90 kDa heat shock protein ATPase | EgrG_000241900.1 | 0,00451208 | 0 | - | - | |
| T-complex protein 1 subunit zeta | gi|576693013 | 0,00625755 | 0 | - | - | |
| 26S proteasome regulatory subunit N11 | EgrG_001166800.1 | 0,00680358 | 0 | - | - | |
| Heat shock protein | gi|576694604 | 0,00444963 | 0,00395170 | 0,0058 | 1,1 | |
| Transitional endoplasmic reticulum ATPase | EgrG_000471600.1 | 0,03403750 | 0,02595500 | 0,024 | 1,3 | |
| Ubiquitin conjugating enzyme | EgrG_000616800.1 | 0,04567950 | 0,03198050 | 0,0043 | 1,4 | |
| Calnexin | EgrG_000875100.1 | 0,00900948 | 0,00611455 | 0,027 | 1,5 | |
| WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2 | gi|576694291 | 0,03321825 | 0,02210675 | 0,042 | 1,5 | |
| Major egg antigen p40 | EgrG_000212700.1 | 0,06261775 | 0,03261750 | 0,00025 | 1,9 | |
| Ubiquilin-1 | gi|576694418 | 0,01723425 | 0,00737003 | 0,00038 | 2,3 | |
| Heat shock protein 60 | EgrG_001190900.1 | 0,11921750 | 0,04692450 | < 0,00010 | 2,5 | |
| Protein AHNAK2 | EgrG_000760400.1 | 0,01505950 | 0,00299023 | < 0,00010 | 5,0 | |
| T complex protein 1 subunit gamma | EgrG_000872100.1 | 0,00943840 | 0,00080528 | 0,0044 | 11,7 | |
| RNA processing and modification | RNA recognition motif RRM domain containing protein | EgrG_000264300.1 | 0,00222925 | 0 | - | - |
| Spliceosome RNA helicase BAT1 | EgrG_000546900.1 | 0,00705580 | 0,00101435 | 0,0082 | 7,0 | |
| Signal transduction mechanisms | Calcium binding protein P22 | EgrG_000447500.1 | 0,04368925 | 0,02742450 | 0,038 | 1,6 |
| Transcription | Serrate RNA effector molecule | gi|576694739 | 0,00042201 | 0 | - | - |
| SNW domain-containing protein | gi|576692125 | 0,00238970 | 0 | - | - | |
| Transcription elongation regulator 1 | EgrG_000862700.1 | 0,00334500 | 0,00144693 | 0,0082 | 2,3 | |
| Elongation factor 1 delta | EgrG_000517100.1 | 0,01624900 | 0,00681798 | < 0,00010 | 2,4 | |
| Translation, ribosomal structure and biogenesis | Aminoacyl tRNA synthase complex interacting | EgrG_001100200.1 | 0,00423773 | 0 | - | - |
| 40S ribosomal protein AS | EgrG_000720100.1 | 0,02209325 | 0,00692395 | 0,00023 | 3,2 | |
| Eukaryotic translation initiation factor 3 | EgrG_000068000.1 | 0,00730010 | 0,00151090 | 0,018 | 4,8 | |
| NSD proteins | ||||||
| Carbohydrate transport and metabolism | Triosephosphate isomerase | EgrG_000416400.1 | 0,01662075 | 0,02560950 | 0,044 | 1,5 |
| Fructose 16 bisphosphate aldolase | EgrG_000905600.1 | 0,03377200 | 0,05451625 | 0,00016 | 1,6 | |
| Cell cycle control, cell division, chromosome partitioning | Rab11 family-interacting protein | gi|576698836 | 0 | 0,0016588 | - | - |
| Structural maintenance of chromosomes protein 2 | EgrG_000602100.1 | 0,0073003 | 0,0101055 | 0,0069 | 1,4 | |
| Translationally controlled tumor protein | EgrG_000058000.1 | 0,0060565 | 0,0223265 | 0,012 | 3,7 | |
| Cell wall/membrane/envelope biogenesis | Ankyrin-3 | gi|576691831 | 0 | 0,00062894 | - | - |
| Cytoskeleton | Protein kinase C and casein kinase substrate in | EgrG_001090800.1 | 0 | 0,00113730 | - | - |
| Ezrin | EgrG_000517000.1 | 0 | 0,00118645 | - | - | |
| Troponin i | EgrG_000734700.1 | 0 | 0,00336850 | - | - | |
| Microtubule associated protein RP:EB family | EgrG_000637300.1 | 0 | 0,00603648 | - | - | |
| Dynein light chain | EgrG_000071300.1 | 0 | 0,02840825 | - | - | |
| Myosin essential light chain, striated adductor muscle | gi|576700259 | 0,11973250 | 0,15936500 | 0,00025 | 1,3 | |
| Spectrin alpha chain | gi|576696380 | 0,00739963 | 0,00999265 | 0,0081 | 1,4 | |
| Myophilin | gi|576693412 | 0,01986200 | 0,02702125 | 0,03 | 1,4 | |
| Tubulin alpha-1C chain | gi|576701165 | 0,01033978 | 0,01517000 | 0,028 | 1,5 | |
| Tropomyosin-2 | gi|576693326 | 0,00569973 | 0,00891180 | 0,035 | 1,6 | |
| Actin modulator protein | EgrG_000882500.1 | 0,01606350 | 0,02763525 | 0,0032 | 1,7 | |
| Gamma aminobutyric acid receptor associated | EgrG_001158000.1 | 0,01022620 | 0,02080425 | 0,0037 | 2,0 | |
| Hematopoietic lineage cell specific protein | EgrG_000936900.1 | 0,00072758 | 0,00487913 | 0,0095 | 6,7 | |
| Cofilin/actin-depolymerizing factor | gi|576697710 | 0,00215878 | 0,02190575 | 0,0012 | 10,1 | |
| Function unknown | Expressed conserved protein | EgrG_000203800.1 | 0 | 0,00223970 | - | - |
| Hypothetical protein | gi|576699770 | 0 | 0,00279715 | - | - | |
| Guanine nucleotide binding protein subunit | EgrG_000200300.1 | 0 | 0,00626868 | - | - | |
| Cystatin B stefin B | EgrG_000159200.1 | 0 | 0,01504050 | - | - | |
| Expressed conserved protein | EgrG_001061900.1 | 0,00958830 | 0,01356800 | 0,03 | 1,4 | |
| Hypothetical protein | gi|576700828 | 0,00681093 | 0,01128425 | 0,0049 | 1,7 | |
| Sj Ts4 protein | EgrG_000393000.1 | 0,01064943 | 0,02080450 | 0,0028 | 2,0 | |
| Hypothetical protein | gi|576692345 | 0,00112804 | 0,00235835 | 0,0058 | 2,1 | |
| Programmed cell death 6 interacting protein | EgrG_000997550.1 | 0,00064300 | 0,00363378 | 0,04 | 5,7 | |
| Hypothetical protein | gi|576690197 | 0,00036507 | 0,00254528 | 0,0052 | 7,0 | |
| Inorganic ion transport and metabolism | Na:K ATPase alpha subunit | EgrG_000342600.1 | 0,00232473 | 0,00436733 | 0,018 | 1,9 |
| Intracellular trafficking, secretion, and vesicular transport | Sorting nexin | EgrG_000922200.1 | 0 | 0,01089525 | - | - |
| Annexin A6 | gi|576697441 | 0,00147718 | 0,00481598 | 0,017 | 3,3 | |
| Post-translational modification, protein turnover, and chaperones | Ubiquitin supergroup | EgrG_001180300.1 | 0 | 0,01742975 | - | - |
| Heat shock protein 71 kDa protein | EgrG_001085100.1 | 0,02868700 | 0,03494425 | 0,048 | 1,2 | |
| Stress induced phosphoprotein 1 | EgrG_000264900.1 | 0,03160575 | 0,03906600 | 0,032 | 1,2 | |
| Thioredoxin peroxidase | EgrG_000791700.1 | 0,05674150 | 0,07183575 | 0,0053 | 1,3 | |
| Dnaj subfamily A | EgrG_000101800.1 | 0,03477350 | 0,04575175 | 0,0057 | 1,3 | |
| Dnaj subfamily B | EgrG_000614200.1 | 0,03305725 | 0,04662200 | 0,0029 | 1,4 | |
| Calreticulin | gi|576693418 | 0,00577333 | 0,00862368 | 0,033 | 1,5 | |
| Calpain | EgrG_000719700.1 | 0,00653558 | 0,01014048 | 0,017 | 1,6 | |
| Ubiquitin-conjugating enzyme E2 L3 | gi|576700841 | 0,00335100 | 0,01493200 | 0,031 | 4,5 | |
| Tumor protein D52 | EgrG_000949820.1 | 0,00238700 | 0,01167500 | 0,0081 | 4,9 | |
| Replication, recombination and repair | Phosphatase 2A inhibitor I2PP2A | EgrG_000465500.1 | 0,02583550 | 0,03972525 | 0,0014 | 1,5 |
| RNA processing and modification | RNA binding protein fox 1 3 | EgrG_000063400.1 | 0 | 0,00132858 | - | - |
| ATP dependent RNA helicase DDXx | EgrG_000098400.1 | 0 | 0,00231058 | - | - | |
| U2 small nuclear RNA auxiliary factor 2 | EgrG_000625400.1 | 0 | 0,00292313 | - | - | |
| Polyadenylate binding protein | EgrG_000689000.1 | 0,00971535 | 0,01186025 | 0,036 | 1,2 | |
| Heterogeneous nuclear ribonucleoprotein A1 | EgrG_000807100.1 | 0,03108225 | 0,04304250 | 0,00081 | 1,4 | |
| Fragile X mental retardation syndrome-related protein | gi|576697665 | 0,00311460 | 0,00595435 | 0,026 | 1,9 | |
| Heterogeneous nuclear ribonucleoprotein K | gi|576694356 | 0,00188858 | 0,00516005 | 0,0063 | 2,7 | |
| Secondary metabolites biosynthesis, transport, and catabolism | ES1 protein mitochondrial | EgrG_000999800.1 | 0 | 0,00349188 | - | - |
| Signal transduction mechanisms | SH2 motif | EgrG_000343500.1 | 0 | 0,00097938 | - | - |
| Tyrosine protein kinase otk | EgrG_000212300.1 | 0 | 0,00210218 | - | - | |
| Nuclear migration protein nudc | EgrG_000463400.1 | 0 | 0,00241293 | - | - | |
| PDZ domain containing protein GIPC3 | EgrG_000190700.1 | 0 | 0,00396823 | - | - | |
| Na:H exchange regulatory cofactor NHE RF2 | EgrG_000743800.1 | 0 | 0,00443958 | - | - | |
| Serine:threonine protein phosphatase PP1 gamma | EgrG_000779500.1 | 0 | 0,00607725 | - | - | |
| Transforming protein RhoA | EgrG_000246600.1 | 0 | 0,00812860 | - | - | |
| Neuronal calcium sensor | EgrG_000186300.1 | 0 | 0,01437175 | - | - | |
| Titin | EgrG_000610500.1 | 0,00034956 | 0,00063859 | 0,009 | 1,8 | |
| Universal stress protein | EgrG_000873800.1 | 0,00811793 | 0,02731575 | 0,00055 | 3,4 | |
| Arfaptin 2 | EgrG_000192300.1 | 0,00156715 | 0,00686460 | 0,039 | 4,4 | |
| Endophilin B2 | EgrG_000060900.1 | 0,00288233 | 0,01315925 | 0,0056 | 4,6 | |
| Transcription | Transcriptional repressor P66-beta | gi|576694405 | 0 | 0,00156535 | - | - |
| Transcription factor AP 4 | EgrG_000768400.1 | 0 | 0,00621390 | - | - | |
| Actin-depolymerizing factor 2 | gi|576697711 | 0,00135023 | 0,00318298 | 0,03 | 2,4 | |
| Translation, ribosomal structure and biogenesis | 40S ribosomal protein S28 | EgrG_001150800.1 | 0 | 0,03077775 | - | - |
| Ribosomal protein LP1 | EgrG_001195100.1 | 0,03494200 | 0,04921800 | 0,015 | 1,4 | |
The proteins that were identified as exclusive in at least two replicates or the proteins that were differentially expressed with significant T-Test values (p≤0.05) are presented.
aFunctional classification determined by eggNOG.
bProtein accession numbers according to GeneDB (www.genedb.org/).
cProtein accession numbers according to NCBI (www.ncbi.nlm.nih.gov/).
dAverage NSAF for SSD replicates.
eAverage NSAF for NSD replicates.