Table 1.
Results of small RNA sequencing. Counts of: total reads from 18–40 nt; reads mapping to the P. striiformis draft genome; reads remaining after uninfected library subtraction; and reads remaining after removing reads matching wheat miRNA and protein-coding genes
| Treatment | IL | IP | UL | UP | Total |
|---|---|---|---|---|---|
| 1a. | |||||
| Total reads | 12,406,438 | 10,462,148 | 16,947,323 | 16,581,382 | 56,397,291 |
| Reads mapping to Pst genome | 190,677 | 176,960 | 196,183 | 185,452 | 749,272 |
| Reads mapping to Pst genome (%) | 1.5 | 1.7 | 1.2 | 1.1 | |
| After subtracting uninfected | 50,909 | 38,362 | -- | -- | 89,271 |
| After filtering | 45,754 | 33,805 | -- | -- | 79,559 |
| 1b. | |||||
| Non-redundant sequences | 3,539,878 | 2,921,622 | 4,982,417 | 4,534,040 | 15,977,957 |
| Sequences mapping to Pst genome | 44,668 | 39,717 | 29,913 | 30,655 | 144,953 |
| Sequences mapping to Pst genome (%) | 1.3 | 1.4 | 0.6 | 0.7 | |
| After subtracting uninfected | 24,107 | 18,843 | -- | -- | 42,950 |
| After filtering | 20,269 | 15,490 | -- | -- | 35,759 |
Treatment-level counts are the sum of three replicates. 1a. Total reads, including redundant reads. 1b. Nonredundant (unique) sequences only
IL Infected Louise, IP Infected Penawawa, UL Uninfected Louise, UP Uninfected Penawawa